This proposal combines in depth investigation of human physiological traits with state-of-art global molecular analyses (omics technology). It concerns aspects to the metabolic action of the hormone insulin, – both when insulin fails to work properly (insulin resistance) and when conditions make insulin work better than normal (insulin sensitization). Our proposal will illuminate molecules that are causing these changes. In this way, we will gain insight to understand insulin resistance – a condition preceding diseases like type 2 diabetes – as well as insight enabling us to enhance insulin sensitivity. We will also explore the value of our analytical platform to be used in stratifying people according to their individual molecular profile (phosphoproteomics) for treatment and prevention strategies – personalized medicine.