More than 10 billion tons of plastic have been manufactured of which >50% are polyolefins, mainly poly -ethylene, -propylene, -styrene and -vinyl chloride. Every year we throw away 200 million of these. The greater part of all plastic waste is incinerated or dumped in landfills, which causes pollution, destroys valuable material resources and leads to the formation of microplastic particles that damage land- and water-based ecosystems. What if plastic waste instead of being waste could be used as a valuable secondary raw material? The proposed project sees plastic waste as a potent carbon source for biomanufacturing of chemicals. However, the tools we have today are harsh depolymerization outside an active site, often dependent on rare metals and leading to a soup of low-value products that are difficult to separate. Opposite PolyFine will enable selective biocatalytic depolymerization of polyolefins inside an active site for plastic-to-chemical recycling under mild conditions.